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Anti-phospho RNA Pol II (Ser2), clone 3E7C7 Antibody
产品别名
Anti-phospho RNA Pol II (Ser2), clone 3E7C7 Antibody
DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription. As the study of the RNA polymerase II (RNAPII) transcription complex expands, many researchers are interested in using immunoblots to detect RNAPII in various protein preparations and column fractions. HeLa cell RNAPII contains 10 subunits, the largest possessing a heptapeptide repeat (PTSPSYS) in the C-terminal domain (CTD). This large subunit cycles, during the course of transcription, through nonphosphorylated (RNAPIIA) and hyperphosphorylated (RNAPIIO) forms. RNAPIIA associates with the basal transcription complex and becomes phosphorylated to the RNAPIIO form before the synthesis of the first phosphodiester bond. The timing of these events suggests that this phosphorylation event may function as a regulatory point, and there is considerable interest in identifying components of the transcription complex with CTD kinase activity. |
Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to human RNA Pol II phosphorylated at Ser2. |
Application【应用】 | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected phospho RNA Pol II (Ser2) in 10 µg of HeLa nuclear extract. Western Blotting: A representative lot detected phospho RNA Pol II (Ser2) in 10 µg of HeLa cell lysate (Odawara, J., et al. (2011). BMC Genomics. 12:516.). Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated phospho RNA Pol II (Ser2) in HeLa cell lysate (Odawara, J., et al. (2011). BMC Genomics. 12:516.; Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.). Chromatin Immunoprecipitation-Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated phospho RNA Pol II (Ser2) in HeLa cell lysate (Odawara, J., et al. (2011). BMC Genomics. 12:516.; Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.). ELISA Analysis: A representative lot from an independent laboratory detected phospho RNA Pol II (Ser2) in a panel of unmodified and modified RNA Pol II (Ser2) proteins (Prof. Taro Tachibana, Cell Engineering Corporation.). Immunocytochemistry Analysis: A 1:500 dilution from a representative lot from an independent laboratory detected phospho RNA Pol II (Ser2) in HeLa cells (Prof. Taro Tachibana, Cell Engineering Corporation.). Alexa Fluor® is a registered trademark of Life Technologies Research Sub Category Transcription Factors Research Category Epigenetics & Nuclear Function This Anti-phospho RNA Pol II (Ser2) antibody, clone 3E7C7 is validated for use in western blotting, ChIP-seq, ELISA, ICC & ChIP for the detection of phospho RNA Pol II (Ser2). |
Quality【质量】 | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho RNA Pol II (Ser2) in 10 µg of HeLa cell lysate. |
Physical form【外形】 | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | ALEXA FLUOR is a registered trademark of Life Technologies |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | rat |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 3E7C7, monoclonal |
species reactivity | monkey, mouse, canine, rat, human |
technique(s) | ChIP: suitable (ChIP-seq) ELISA: suitable immunocytochemistry: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2aκ |
NCBI accession no.【NCBI登记号】 | NP_000928 |
UniProt accession no.【UniProt登记号】 | P24928 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~217 kDa observed |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |