Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody

32160702

NA.41

There are two kinds of IGF receptors based on their relative affinities for IGF-1 versus IGF-2. The IGF-1 receptor (IGF-1R) prefers IGF-1 over IGF-2 and weakly binds insulin. IGF-1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kDa) and two beta (95 kDa) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF-1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF-1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by mediating signaling pathways that enhance cell survival.

This antibody recognizes IGF-1R phosphorylated at Tyr1161/Tyr1165/Tyr1166. Please note that some sources list the phsophorylation sites as Tyr1131/Tyr1135/Tyr1136. The difference in the amino acid sequence is due to a 30 amino acid signal chain that is removed due to protein processing.

KLH-conjugated linear peptide corresponding to human IGF-1R phosphorylated at Tyr1161/Tyr1165/Tyr1166.
Epitope: Phosphorylated Tyr1161/Tyr1165/Tyr1166.

Research Sub Category
Insulin/Energy Signaling

Insulin/Energy Signaling
Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody is an antibody against phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) for use in WB, Cell Function Assay.
Western Blot Peptide Inhibition Analysis: A representative lot detected IGF-1R in IGF1 treated HEK293 cell lysate.
Research Category
Epigenetics & Nuclear Function

Signaling

Evaluated by Western Blot in IGF1treated HEK293 cell lysate.

Western Blot Analysis: A 1:200 dilution of this antibody detected IGF-1R in IGF1 treated HEK293 cell lysate.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
IGF1 untreated and treated HEK293 cell lysate

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, pig

rat (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology), Xenopus (based on 100% sequence homology), porcine (based on 100% sequence homology), chicken (based on 100% sequence homology)

cell based assay: suitable
western blot: suitable

NP_000866

P08069

wet ice

human ... IGF1R(3480)

~95 kDa observed. IGF-1R can be cleaved into an Insulin-like growth factor 1 receptor alpha (aa 31-736) and beta (aa 741-1367) chain. Both chains are known to contain multiple potential glycosylation sites. Distinct beta-subunits are present in hybrid insulin-like-growth-factor-1 receptors in the central nervous system. (Moss, A.M., et al. (1993). Biochem J. 294( Pt 3):685-692.)

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1