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Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12
产品别名
Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12
DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription. |
| Immunogen【免疫原】 | Ovalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Tyr1. |
| Application【应用】 | Immunoprecipitation Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.). Detect the phospho-RNAPII (Tyr1) protein using this Anti-phospho-RNAPII (Tyr1), clone 3D12 validated for use in WB, IP & ChIP. |
| Quality【质量】 | Evaluated by Western Blot Analysis in untreated and lambda phosphatase treated HeLa cell lysates. Western Blot Analysis: 2 µg/mL of this antibody detected phospho-RNAPII (Tyr1) in 10 µg of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.) |
| Physical form【外形】 | Protein G Purified Format: Purified |
| Analysis Note【分析说明】 | Control Untreated and lambda phosphatase treated HeLa cell lysates |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | rat |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 3D12, monoclonal |
| species reactivity | human |
| technique(s) | ChIP: suitable immunoprecipitation (IP): suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG1κ |
| NCBI accession no.【NCBI登记号】 | NP_000928 |
| UniProt accession no.【UniProt登记号】 | P24928 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~220 kDa observed |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
