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Anti-Prion Protein Antibody, a.a. 109-112, clone 3F4
产品别名
Anti-Prion Protein Antibody, a.a. 109-112, clone 3F4
PrP, CD230
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Prions are thought to cause a number of diseases in a variety of mammals, including bovine spongiform encephalopathy (BSE, also known as "mad cow disease") in cattle and the Creutzfeldt-Jakob disease (CJD) in humans. All thus-far hypothesized prion diseases affect the structure of the brain or other neural tissue, and all are currently untreatable and thought to be fatal. Prions are hypothesized to infect and propagate by refolding abnormally into a structure which is able to convert normal molecules of the protein into the abnormally structured form. All known prions induce the formation of an amyloid fold, in which the protein polymerises into an aggregate consisting of tightly packed beta sheets. This altered structure is extremely stable and accumulates in infected tissue, causing cell death and tissue damage. This stability means that prions are resistant to denaturation by chemical and physical agents, making disposal and containment of these particles difficult. |
Specificity【特异性】 | Prion protein, amino acid residues 109-112 of human, hamster and feline. Does not react with PrP from any other mammalian species. MAB1562 is reactive to native and denatured forms of PrP. Tissue or cells which have been fixed requires that the epitope be re-exposed (see below). Recognizes both protease sensitive and protease resistant forms of PrP. |
Immunogen【免疫原】 | Epitope: a.a. 109-112 |
Application【应用】 | Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Brain Immunohistochemistry (Kitamoto et al., 1987): 1:100-1:1,000 *See protocol below. Epitope must be re-exposed in fixed tissue by pretreatment of tissue using one of the following procedures: a. formic acid for 10 minutes at room temperature (Kitamoto et al., 1987) b. hydrolytic autoclaving (Kitamoto et al., 1991) c. microwaving (BioGenex, San Ramon, CA) Western Blot: (Kascsak, R.J., 1991; Kascsak, R.J., 1987): 1:10,000-1:100,000 dilution of a previous lot was used. Immunoprecipitation: (Kascsak, R.J., 1991; Kascsak, R.J., 1987): 1:10-1:100 dilution of a previous lot was used. ELISA: (Kascsak, R.J., 1991; Kascsak, R.J., 1987): Optimal working dilutions must be determined by end user. This Anti-Prion Protein Antibody, a.a. 109-112, clone 3F4 is validated for use in ELISA, IH, IH(P), IP, WB for the detection of Prion Protein. |
Quality【质量】 | Immunohistochemistry(paraffin): Prion Protein (cat. # MAB1562) staining pattern/morphology in normal brain. Tissue was pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:500, using IHC-Select Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Brain |
Physical form【外形】 | Purified mouse monoclonal IgG2a in buffer containing PBS and no preservative. Protein A purified Format: Purified |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
clone【克隆】 | 3F4, monoclonal |
species reactivity | human, hamster |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2a |
NCBI accession no.【NCBI登记号】 | NM_000311.3 |
UniProt accession no.【UniProt登记号】 | P04156 |
shipped in【运输】 | dry ice |
Gene Information | human ... PRNP(5621) |
产品说明
Target description【目标描述】 | 12.3 kDa |
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |