Anti-RAG-1 Antibody

32160702

NA.41

V(D)J recombination-activating protein 1 (RAG-1) is also called RING finger protein 74. RAG-1 is a component of the RAG complex, which mediates the DNA cleavage phase during V(D)J recombination. As part of the RAG complex, RAG1 mediates the DNA-binding and catalyzes the DNA cleavage activities by introducing a double-strand breaks between the recombination signal sequences (RSS) and the adjacent coding segment. RAG-1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3 as well as polyubiquitination of KPNA1. RAG-1 is expressed in maturing lymphoid cells. RAG-1 defects can cause disease states such as: combined cellular and humoral immune defects with granulomas (CHIDG), severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive (T-B-NK+ SCID), Omenn syndrome (OS), and alpha/beta T-cell lymphopenia with gamma/delta T-cell expansion severe cytomegalovirus infection and autoimmunity (T-CMVA).

KLH-conjugated linear peptide corresponding to a region near the N-terminus of human RAG-1.

Detect RAG-1 using this rabbit polyclonal antibody, Anti-RAG-1 Antibody validated for use in western blotting, ICC & IP.
Western Blotting Analysis: 1 µg/mL from a representative lot detected RAG-1 immunopreciptated from 500 µg of HL-60 cell lysate. (Note: It is recommended to immunoprecipitate RAG-1 before performing WB).

Immunocytochemistry Analysis: 1:100 dilution from a representative lot detected RAG-1 in Jurkat cells.

DyLight^® is a registered trademark of Thermo Fisher Scientific.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Evaluated by Immunoprecipitation in HL-60 cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated RAG-1 in 500 µg of HL-60 cell lysate. Immunopreciptated protein was then probed with 1 µg/mL of the same antibody in WB.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

DyLight is a registered trademark of Pierce Biotechnology, Inc.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NP_000439

P15918

wet ice

human ... RAG1(5896)

~120 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1