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Lin28-TAT human
| 产品编号： | 4103022 |
| 规格： | recombinant, expressed in E. coli, ≥90% (SDS-PAGE), ≥90% (HPLC), suitable for cell culture |
| 包装规格： | 25 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Lin28-TAT human
ZCCHC1
CSDD1
LIN28A
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | Lin28 is a RNA-binding protein that belongs to a diverse family of structurally-related transcription factors. Lin28 is found abundantly in embryonic stem cells (ESCs), and to a lesser extent in placenta and testis. Lin28 has been shown to block let-7 microRNA processing and maturation, a necessary step in the differentiation of stem cells and certain cancer cell lines. Together with Sox2, Oct4, and Nanog, Lin28 can induce the reprogramming of primary human fibroblasts to a pluripotent state. Lin28 and other regulatory proteins can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing proteins into primary as well as transformed cells. Recombinant human Lin28-TAT is a 24.4 kDa protein containing 222 amino acid residues, including 13- residue C-terminal TAT peptide. |
| Biochem/physiol Actions【生化/生理作用】 | Lin28 is a RNA-binding protein that belongs to a diverse family of structurally-related transcription factors. Recombinant human Lin28-TAT is a 24.4 kDa protein containing 222 amino acid residues, including 13- residue C-terminal TAT peptide. |
| Sequence【序列】 | GPSVSNQQFA GGCAKAAEEA PEEAPEDAAR AADEPQLLHG AGICKWFNVR MGFGFLSMTA RAGVALDPPV DVFVHQSKLH MEGFRSLKEG EAVEFTFKKS AKGLESIRVT GPGGVFCIGS ERRPKGKSMQ KRRSKGDRCY NCGGLDHHAK ECKLPPQPKK CHFCQSISHM VASCPLKAQQ GPSAQGKPTY FREEEEEIHS PTLLPEAQNG GYGRKKRRQR RR |
| Physical form【外形】 | Lyophilized from 10 mM Sodium Citrate, pH 3.0. |
| Reconstitution【重悬】 | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | ≥90% (HPLC) ≥90% (SDS-PAGE) |
| form【形式】 | lyophilized |
| mol wt【分子量】 | 24.4 kDa |
| packaging【包装】 | pkg of 25 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities【杂质】 | <0.1 EU/μg endotoxin, tested |
| color【颜色】 | white to off-white |
| UniProt accession no.【UniProt登记号】 | Q9H9Z2 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... LIN28A(79727) |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
