α-L-Fucosidase Assay Kit

100

colorimetric

cancer, diabetes; endocrinological disorders, hepatitis; gastrointestinal diseases

2-8℃

The kit is suitable for the quantitative determination of α-L-Fucosidase activity in plasma, serum, tissue, cell lysate, etc.

α-L-Fucosidase (AFU) is an enzyme coded by the FUCA1 gene in humans and catalyzes the breakdown of L-Fucose. A genetic deficiency in this enzyme results in a neurovisceral storage disease, fucosidosis, which is characterized by the accumulation of fucose. Low serum activity of fucosidase has also been linked to ovarian caricinoma. Elevated fucosidase serum activity has been observed in patients with diabetes, hyperthyroidism, cirrhosis, and hepatitis. Increased activity has been associated with lung, breast, stomach, ovary, uterus, and liver carcinomas.

The non-radioactive, colorimetric α-L-Fucosidase Assay Kit is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at A405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

GHS07

Warning

H319

P264 - P280 - P305 + P351 + P338 - P337 + P313

Eye Irrit. 2

10 - Combustible liquids

WGK 2