Anti-Actin Antibody, clone C4, Alexa Fluor^® 555 Conjugate

32160702

NA.41

Actins are ubiquitous eukaryotic proteins that serve as a multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, trascriptional regulation and vesicle trafficking. These funstions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do not show spatial, temporal and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Beta and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure. Exocytosis, and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. Alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. It has been shown that under calcium-bound conditions, beta-actin exhibits more dynamic behavior than gamma-actin with higher rates of polymerization and depolymerization. Also, beta- and gamma-actins can readily copolymerize, and the resulting filaments exhibit polymerization and depolymerization rates that vary depending on the ration of beta- to gamma-actin (Lessard, JL.,et al.(1988). Cell Motility Cytoskeleton 10(3); 349-362.

To date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.
MAB1501 is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts. Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).

Purified chicken gizzard actin (Lessard, 1988).
Epitope is conserved in all known actins.

Research Category
Cell Structure
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Actin in A431, and NIH/3T3 cells.
The unconjugated version of this antibody (Cat. No. MAB1501) has been demonstrated to perform in WB, ELISA, ICC, IF, IHC, IH(P).
This Anti-Actin Antibody, clone C4, Alexa Fluor^® 555 Conjugate is validated for use in Immunocytochemistry for the detection of Actin.
Research Sub Category
Adhesion (CAMs)

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Actin in HeLa cells.

Purified mouse antibody conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1% sodium azide.
Protein G Purified

Concentration: Please refer to lot specific datasheet.

ALEXA FLUOR is a registered trademark of Life Technologies

100

mouse

ALEXA FLUOR^® 647

purified antibody

primary antibodies

C4, monoclonal

human

chicken (based on 100% sequence homology)

immunocytochemistry: suitable

IgG2bκ

NP_001026234.1

P68139

wet ice

human ... ACTA1(58)

40 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

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10 - Combustible liquids

WGK 2