Anti-Aggrecan Antibody, MMP Cleaved, NT FFGVG neoepitopes, clone AF-28

32160702

NA.41

Aggrecan is also known as aggregating chondroitin sulphate proteoglycan. Aggrecan is the major proteoglycan present in articular cartilage, composing up to 10% of its dry weight. It is responsible for endowing articular with its intrinsic properties of load bearing and compressive forces.

Mouse anti-aggrecan is a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments. The antibody recognizes neo-epitopes on polypeptides with N-terminal FFGVG sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with MMPs. By immunoblotting, AF-28 specifically detected G2 fragments derived from an aggrecan G1-G2 substrate digested with stromelysin, collagenase, gelatinase, and matrilysin, but failed to detect G2 fragments obtained from elastase, trypsin, or cathepsin B digests. Undigested G1-G2 was not detected. Competition experiments confirmed that peptides containing internal FFGVG sequences were not detected by the antibody. Clone AF-28 specifically recognizes a neo-epitope on polypeptides with N-terminal FFGVG Sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with matrix metalloproteinases.

FFGVGGEEDC-KLH peptide
Epitope: N-terminus FFGVG neoepitopes

Research Category
Cell Structure
Western blot

ELISA

Optimal working dilutions must be determined by the end user.
Research Sub Category
ECM Proteins

Inflammation & Autoimmune Mechanisms
Detect Aggrecan using this Anti-Aggrecan Antibody, MMP Cleaved, N-terminus FFGVG neoepitopes, clone AF-28 validated for use in ELISA & WB.

Liquid in PBS pH 7.4 containing 0.09% sodium azide as a preservative.
Format: Purified
Protein A purified

Control
Cartilage, neural tube, and brain tissue

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified antibody

primary antibodies

AF-28, monoclonal

bovine, human, rat, mouse, pig

guinea pig, horse

Chemicon^®

ELISA: suitable
western blot: suitable

IgG1

NM_001135.2
NM_013227.2

P16112

wet ice

human ... ACAN(176)

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2