Anti-Ambra1 Antibody

32160702

NA.41

Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) is a developmental protein that is involved in autophagy, differentiation and nuerogenesis. AMBRA1 regulates autophagy and development of the nervous system, normal cell survival and proliferation, and the control of protein turnover during neuronal development.

This antibody recognizes the C-terminus of Ambra1. This antibody should recognize isoforms 1 through 5 based upon immunogen design.

Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of mouse Ambra1.

Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
Western Blotting Analysis: 0.5 µg/mL of a representative lot detected Ambra1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of a representative lot detected Ambra1 in human cell lysates (HeLa & U87MG) and in mouse tissue homogenates (liver, kidney, and embryo), as well as exogenously expressed myc-Ambra1 in lysates from transfected HEK293 cells. (Courtesy of Dr. Sabrina Di Bartolomeo, Cecconi Lab at the Dulbecco Telethon Institute, Italy).
Western Blotting Analysis: Target specificity of a representative lot was confirmed by Western blotting analysis of Ambra1-knock mouse embryos and HeLa cells treated with Ambra1 siRNA. (Courtesy of Dr. Sabrina Di Bartolomeo, Cecconi Lab at the Dulbecco Telethon Institute, Italy).
Western Blotting Analysis: A representative lot detected decreased Ambra1 expression in tissue homogenates (kidney, liver, and lung) from Ambra1+/- mice when compared with tissue samples from wild-type mice. (Cianfanelli, V., et al. (2015). Nat. Cell Biol. 17(1):20-30).
Anti-Ambra1 Antibody is a rabbit polyclonal antibody, validated for use in western blotting.

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Ambra1 in 10 µg of MCF7 cell lysate.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
MCF7 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

mouse, human

rat (based on 100% sequence homology)

western blot: suitable

NP_7662587

A2AH22

wet ice

human ... AMBRA1(55626)

~140 kDa observed. Uniprot describes 7 isoforms at 143 kDa (isoform 1), 133 kDa (isoform 2), 87 kDa (isoform 3), 84 kDa (isoform 4), 100 kDa (isoform 5), 136 kDa (isoform 6), and 130kDa (Isoform 7). Uncharacterized band(s) may be observed in some cell lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1