Anti-Annexin VI Antibody, clone 4B11.1

32160702

NA.41

The protein Annexin A6 or alternatively known as 67 kDa calelectrin, Annexin VI, Annexin-6, Calphobindin-II (CPB-II), Chromobindin-20, Lipocortin VI, Protein III, p68, or p70 and encoded by the human gene named ANXA6 or ANX6. Annexin VI is part of a large family of Annexin proteins and shares with the family the ability to bind negatively charged phospholipids in a calcium dependent manner. Localized in the plasma membrane annexins are important in many cellular functions as the provide scaffolds and play roles in vesicle transport, exocytosis, endocytosis and calcium transport and conduction. Recent research indicates that Annexin VI can interact with PKCalpha and EGFR in A431 cells to help regulate the phosphorylation of pY-EGFR and the associated activation of downstream signaling events associated with cancer. Annexin VI in normal tissue is expressed most strongly in myocardial tissue where it associates with sarcomeric α-actinin and is part of the sarcomere structure. Annexin VI also regulates terminal chondrocyte differentiation by modulating key signaling pathway activities.

GST-tagged recombinant protein corresponding to human Annexin VI.

Western Blotting Analysis: 1 µg/mL from a representative lot detected Annexin VI in 10 µg of C2C12 cell lysate.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Annexin VI in HeLa and A431 cells.

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Research Sub Category
Apoptosis - Additional
This Anti-Annexin VI Antibody, clone 4B11.1 is validated for use in western blotting & ICC for the detection of Annexin VI.
Research Category
Apoptosis & Cancer

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Annexin VI in 10 µg of SH-SY5Y cell lysate.

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

ALEXA FLUOR is a registered trademark of Life Technologies

100

mouse

purified immunoglobulin

primary antibodies

4B11.1, monoclonal

human, mouse

immunocytochemistry: suitable
western blot: suitable

IgG2aκ

NP_001146

P08133

wet ice

human ... ANXA6(309)

~75 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1