Anti-ASF-1 (SF2) Antibody, clone AK96

32160702

NA.41

Alternative-splicing factor 1 (ASF-1), also known as SRp30a or SF2, is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase.

This antibody recognizes the N-terminus of ASF-1 (SF2).

MBP-tagged recombinant protein corresponding to the N-terminus of human ASF-1 (SF2).
Epitope: N-terminus

Use Anti-ASF-1 (SF2) Antibody, clone AK96 (mouse monoclonal antibody) validated in WB, IP, ICC, IHC to detect ASF-1 (SF2) also known as splicing factor arginine/serine-rich 1, Alternative-splicing factor 1 & pre-mRNA-splicing factor SF2 P33 subunit
Research Category
Epigenetics & Nuclear Function
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ASF-1 (SF2) in human prostate carcinoma cells and human testis tissue.
Western Blotting Analysis: A representative lot was used by an independent laboratory in western blotting (Sinha, R., et al. (2010). Mol. Cell. Biol. doi:10.1128/MCB.01270-09).
Immunoprecipitation Analysis: A representative lot immunoprecipitated ASF-1 (SF2) in HeLa nuclear extracts (Jablonski, J. A., et al. (2010). Nucleic Acids Research. 38(4):1249-1260).
Research Sub Category
RNA Metabolism & Binding Proteins

Evaluated by Western Blot in human lymph node lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected ASF-1 (SF2) on 10 µg of human lymph node lysate.

Replaces: MABE512

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without 0.05% sodium azide.
Protein G Purified

Control
Human lymph node lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

AK96, monoclonal

human

rat (immunogen homology), mouse (immunogen homology)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG2bκ

NP_001071634

Q07955

wet ice

~ 30 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1