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Anti-β-Catenin antibody, Mouse monoclonal
| 产品编号： | 4105271 |
| 规格： | clone 15B8, purified from hybridoma cell culture |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-β-Catenin antibody, Mouse monoclonal
Anti-armadillo
Anti-CTNNB1
基本信息
| NACRES | NA.41 |
| General description【一般描述】 | Anti-β-Catenin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant chicken β-Catenin. β-Catenin, also known as Catenin βfamily. |
| Immunogen【免疫原】 | recombinant chicken ß-Catenin |
| Application【应用】 | Anti-β-Catenin antibody, Mouse monoclonal has been used in:
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| Biochem/physiol Actions【生化/生理作用】 | β-Catenin plays a dual function in the cell including cell-cell adhesion and as a component of the Wnt (wingless) signaling pathway. In adherens junctions, β-Catenin associates with the cytoplasmic domain of the cadherin family adhesion receptors, anchoring them to the actin cytoskeleton and thus acting as structural protein. β-Catenin interacts with T cell factor (TCF)- family transcription factors to activate target genes. Wnt/β-catenin signaling interacts with several cellular pathways in context-dependent ways, including JakSTAT (Janus kinase−Signal transducer and activator of transcription) cytokine, TGFβ (transforming growth factor−β), DNA damage and the Hippo signaling pathways. Furthermore, β-Catenin is involved in embryonic morphogenesis and tumorigenesis and is tightly regulated by phosphorylation and further degradation by the ubiquitin-proteasome system. |
| Physical form【外形】 | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
产品性质
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone【克隆】 | 15B8, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~94 kDa |
| species reactivity | rat, bovine, monkey, chicken, mouse, human, hamster, canine |
| concentration【浓度】 | ~1.0 mg/mL |
| technique(s) | immunoblotting: 0.25-0.5 μg/mL using Madin-Darby bovine kidney (MDBK) cell line extract immunofluorescence: 5-10 μg/mL using human breast adenocarcinoma MCF7 cells immunohistochemistry: suitable immunoprecipitation (IP): suitable |
| isotype【同位素/亚型】 | IgG1 |
| UniProt accession no.【UniProt登记号】 | P35222 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
