Anti-Chymase Antibody, clone B7

32160702

NA.41

Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.

Reacts with human mast cell chymase.

Chymase purified from human skin.

Research Sub Category
Inflammation & Autoimmune Mechanisms
Dot blotting: 0.5-1.0 μg/mL.

Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

Not recommended for use on Western blots.

Optimal working dilutions must be determined by end user.
Anti-Chymase Antibody, clone B7 is an antibody against Chymase for use in DB, IH.
Research Category
Inflammation & Immunology

Purified by protein G affinity chromatography. Liquid in PBS with 20% glycerol, pH 7.4.
Format: Purified

Control
POSITIVE CONTROL: lung, heart, skin, placenta.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

affinity isolated antibody

primary antibodies

B7, monoclonal

human

Chemicon^®

2.4 mg/mL

dot blot: suitable
immunohistochemistry: suitable

IgG1κ

not suitable for Western blot

NM_001836.2

P23946

wet ice

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. Antibody may also be stored at -20ºC in undiluted aliquots.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1