Anti-CRTC2 Antibody, clone 5B10

32160702

NA.41

CREB-regulated transcription coactivator 2 (CRTC2) is a transcriptional coactivator for CREB1 which is a cAMP (CRE) site responder. CRTC2 is also known as TORC-2 (transducer of regulated cAMP response element-binding protein 2) and CRTC2 regulates gluconeogenesis as well as steroidogenic processes, and is also a coactivator for mitochondrial biogenesis in muscle cells. Localization is cytoplasmic but relocates to the nucleus in response to cAMP and glucagon levels. CRTC2 is highly expressed in thymus, spleen, muscle, and lung; it is also expressed in various cancers as well as insulinomas. Low levels of expression are found in the brain, colon, heart and kidney, with the lowest levels being in the liver and pancreas. Activity is regulated via phosphorylation. CRTC2 is inactive when phosphorylated (meaning that is does not bind CREB and assist in gene transcription) and active when dephosphorylated. EMD-Millipore’s Anti-CRTC2 mouse monoclonal, clone 5B10, has been tested in western blots against human HEK292 and HeLa cell extracts, in immunohistochemistry in paraffin embedded human ovary and lung cancer tissue, and in immunofluorescent immunocytochemistry and flow cytometry on HeLa cells in culture.

Purified protein fragment of human CRTC2 expressed in E. coli.

Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CRTC2 in HeLa cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CRTC2 in human lung cancer and ovarian tumor tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CRTC2 in HeLa cells.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Anti-CRTC2 Antibody, clone 5B10 is a highly specific mouse monoclonal antibody, that targets CREB-regulated transcription coactivator 2 & has been tested in western blotting, ICC, IHC & Flow Cytometry.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CRTC2 in 10 µg of HeLa cell lysate.

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Unpurified

Control
HeLa cell lysate

100

mouse

ascites fluid

primary antibodies

5B10, monoclonal

human

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

IgG1

NP_859066

Q53ET0

wet ice

human ... CRTC2(200186)

~76 kDa observed.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

nwg