Anti-Cytochrome P450 (scc) Antibody, a.a. 509-526

32160702

NA.41

As a member of the cytochrome P450 family, Cytochrome P450 Side Chain Cleavage Enzyme (scc) catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone during the first step of steroid biosynthesis. It is expressed in certain organs, such as ovaries, adrenal glands, and testis. Deficiency in CYP11A1 is commonly associated with adrenal insufficiency, and with variable degrees of disorder of sex development (DSD).

This antibody recognizes the C-terminus of Cytochrome P450 (scc), a.a. 509-526.

KLH-conjugated linear peptide corresponding to the C-terminus of rat Cytochrome P450 (scc), a.a. 509-526.

Immunohistochemistry Analysis: A 1:4,000-8,000 dilution from a representative lot detected Cytochrome P450 (scc) in rat adrenal gland and rat testis tissue.
This Cytochrome P450 (scc) antibody is validated for use in WB & IHC for the detection of the Cytochrome P450 (scc) protein.

Evaluated by Western Blot in rat adrenal tissue lysate.

Western Blot Analysis: 0.02 µg/mL of this antibody detected Cytochrome P450 (scc) in 10 µg of rat adrenal tissue lysate.

Replaces: AB1294

Affinity purified

Control
Rat adrenal tissue lysate

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

rat

immunohistochemistry: suitable (paraffin)
western blot: suitable

NP_058982

P14137

wet ice

~52 kDa observed. On higher percentage gels and longer exposures, 8 kDa cleavage product can be detected.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1