Anti-Cytochrome P450 11β-Hydroxylase Antibody, clone 11B2-1A4

32160702

NA.41

Rat p450 11beta-Hydroxylase

Aa 272-285 (KNVYRELAE GRQQS-C)

Anti-Cytochrome P450 11β-Hydroxylase Antibody, clone 11B2-1A4 is an antibody against Cytochrome P450 11β-Hydroxylase for use in IH(P) & WB.
Western blot: 1:300

Immunohistochemistry (S.T.F. on paraffin embedded tissue required, traditional formalin fixation or paraformaldehyde fixation is not recommended. Microwave or pressure cooker antigen retrieval; blocking with a solution of 0.5% SDS, 2% milk and 5% NGS in Tris-HCL pH 7.6. Primary antibody is diluted in PBS or TBS typically with no SDS or NGS: 1:50-200, usually overnight refrigerated. After washing 4X with Tris buffer pH 7.6, secondary goat anti-mouse secondary (AP124B) is incubated (1:500-1:2000) diluted in a solution of 2% milk, 5% NGS, Tris-HCL pH 7.6 and 0.05% tween 20. Detection is via streptavidin HRP/DAB. Recommended counter stain is Gill′s Haematoxylin.

Optimal working dilutions must be determined by end user.

STF fixative is available from streck laboratories, www.streck.com).
Research Category
Metabolism
Research Sub Category
Enzymes & Biochemistry

Supernatant. Liquid in 0.1% sodium azide.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

culture supernatant

primary antibodies

11B2-1A4, monoclonal

rat

Chemicon^®

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG1

P30099

wet ice

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

nwg