Anti-Cytochrome P450 Aldosterone Synthase Antibody, clone 1G5

32160702

NA.41

Aldosterone synthase (or 18-hydroxylase) is a steroid hydroxylase cytochrome P450 oxidase enzyme involved in the generation of aldosterone. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. (Pascoe, L, et al. (1992) "Mutations in the human CYP11B2 (aldosterone synthase) gene causing corticosterone methyloxidase II deficiency." PNAS, USA. 89:496-5000).

Rat cytochrome p450 aldosterone synthase

Aldo-1-MAP (aa 175-192) (KVRQNARGSLTMDVQQSL-MAP)

Western blot: recognizes ~48kDa band in adrenal zona glomerulosa mitochondrial preparations. The enzyme is expressed best in adrenal zona glomerulosa tissues, expression in kidney is virtually undetectable.
Immunohistochemistry: 1:50-1:200 , ABC, enzymatic detection; clone works best with STF fixed tissues that are paraffin embedded. STF is available from Streck Laboratories, www.streck.com. Formalin fixation has been successfully reported but working dilutions are much lower (see Fujisawa, et al). Recommended counter stain is Gill′s Haematoxylin.
Immunocytochemistry: acetone fixed cells, 1:20-1:200.
ELISA
Optimal working dilutions must be determined by end user.

This Anti-Cytochrome P450 Aldosterone Synthase Antibody, clone 1G5 is validated for use in ELISA, IC, IH(P), WB for the detection of Cytochrome P450 Aldosterone Synthase.

Unpurified tissue culture supernatant containing 50% glycerol and 0.1% sodium azide as a preservative.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

culture supernatant

primary antibodies

1G5, monoclonal

rat, mouse

Chemicon^®

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG1κ

P30099

wet ice

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10 - Combustible liquids

WGK 1