Anti-EphB2 Antibody, clone 2D12C6

32160702

NA.41

Ephrin type-B receptor 2 (EphB2) is a receptor tyrosine kinase for ephrin ligands that mediate numerous developmental processes, particularly in the nervous system. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB2 is critical for embryonic/postnatal development for lateral suprapyramidal blade formation which is in a specific region of the dentate gyrus. EphB2 is expressed in the two main components of the thymus, developing thymocytes, TEC, colorectal cancer, and in the earliest SGZ progenitor cells as they migrate during development and form the dentate gyrus.

Purified recombinant fragment of EphB2 expressed in E. coli.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected EphB2 in HeLa and HepG2 cells.

Immunohistochemistry Analysis: 1:200 to 1:1,000 dilution from a representative lot detected EphB2 in rat cerebellum, pons/midbrain, and hippocampus tissues. Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Anti-EphB2 Antibody, clone 2D12C6 is a highly specific mouse monoclonal antibody, that targets EphB2 & has been tested in western blotting, IHC & ICC.

Evaluated by Western Blot in IMR-32 cell lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected EphB2 in 10 µg of IMR-32 cell lysate.

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Unpurified

Control
IMR-32 cell lysate

100

mouse

ascites fluid

primary antibodies

2D12C6, monoclonal

human, rat

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

IgG2b

Q6NVW1

wet ice

human ... EPHB2(2048), EphB2(2048)
rat ... EphB2(313633)

~53 kDa observed

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

nwg