Anti-Excitatory amino acid transporter 2 Antibody, clone G6

32160702

NA.41

The activity of the main glutamate transporter in the CNS, Excitatory amino acid transporter 2 (also known as GLT1), can be regulated by protein kinase C (PKC). It is known that activation of PKC by phorbol esters promotes the clathrin-dependent internalization of the transporter, followed by its lysosomal degradation. However, the molecular mechanisms that link PKC activation and the internalization of the excitatory amino acid transporter 2 are not fully understood. The internalization process may play a role in the ubiquitylation of lysine residues located in the C-terminal tail of the excitatory amino acid transporter 2. Exposure to PMA increases the ubiquitylation of the excitatory amino acid transporter 2 in transfected cells and in the rat brain, and this ubiquitylated excitatory amino acid transporter 2 accumulates in the intracellular compartment. The activation of PKC induces the ubiquitylation of these C-terminal lysine residues in the Excitatory amino acid transporter 2 and this modification mediates the interaction of the transporter with the endocytic machinery.

This antibody recognizes the C-terminus of Excitatory amino acid transporter 2.

Linear peptide corresponding to the C-terminus of human Excitatory amino acid transporter 2.
Epitope: C-terminus

Western Blot Analysis: 0.5 µg/ml from a previous lot detected Excitatory amino acid transporter 2 on 10 µg of human brain tissue lysate.
Research Sub Category
Signaling Neuroscience
Research Category
Neuroscience
This Anti-Excitatory amino acid transporter 2 Antibody, clone G6 is validated for use in WB, IH, IC for the detection of Excitatory amino acid transporter 2.

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Excitatory amino acid transporter 2 on 10 µg of mouse brain tissue lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
Mouse brain tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

G6, monoclonal

human, mouse

rat (100% immunogen homology)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

IgG1κ

NP_004162

P43004

wet ice

human ... SLC1A2(6506)

62 kDa

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1