Anti-EZH2 Antibody, clone AE25

32160702

NA.41

EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. EZH2 is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis, and placenta. EZH2 and BMI-1 genes are coexpressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene.

MBP-tagged recombinant protein corresponding to human EZH2.

Western Blot Analysis: A 1:500 dilution from a representative lot detected EZH2 in Jurkat and HeLa cell lysates.

Immunohistochemistry Analysis: A representative lot detected EZH2 in breast carcinoma tissue (Pietersen et al., 2008)
Research Category
Epigenetics & Nuclear Function
This Anti-EZH2 Antibody, clone AE25 is validated for use in Western Blotting, IHC for the detection of EZH2.
Research Sub Category
Chromatin Biology

Evaluated by Western Blot in T47D cell lysate.

Western Blot Analysis: A 1:1000 dilution of this antibody detected EZH2 in 10 µg of T47D cell lysate.

Replaces: 04-1047

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
T47D cell lysate

100

mouse

purified antibody

primary antibodies

AE25, monoclonal

human

immunohistochemistry: suitable
western blot: suitable

IgG1κ

NP_001190176

Q15910

wet ice

~80 kDa observed. Uniprot describes multiple isoforms ranging from 80-86 kDa

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1