Anti-FTO Antibody, clone 5-2H10

32160702

NA.41

Alpha-ketoglutarate-dependent dioxygenase FTO (Fat mass and obesity-associated protein) is a ubiquitous nuclear protein which belongs to the FTO family. It is a dioxygenase enzyme that dealkylates nucleic acids by oxidative demethylation in the presence of oxygen, alpha-ketoglutarate and iron (Fe2+). FTO is highly active on single-stranded RNA molecules with 3-methyluracil residues and on single-stranded DNA containing 3-methylthymine residues, and has relatively low activity on single-stranded DNA containing either 3-methylcytosine or 3-methyladenine. There has been no observed activity on double-stranded DNA. FTO is highly abundant in the hypothalamus, pituitary, and adrenal glands. It plays an important role in energy homeostasis and the regulation of fat accumulation in the body, and may be involved in obesity. Mutated FTO proteins are linked to a syndrome characterized by retarded growth, delayed development, coarse facies and early death.

Recombinant protein corresponding to human FTO.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected FTO in glandular cells of rat pituitary gland tissue.
Anti-FTO Antibody, clone 5-2H10 detects level of FTO & has been published & validated for use in Western Blotting, IHC(P).
Research Sub Category
Cell Cycle, DNA Replication & Repair
Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blot in human kidney tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected FTO in 10 µg of human kidney tissue lysate.

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

Control
Human kidney tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

5-2H10, monoclonal

human, rat

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG2aκ

NP_001073901

Q9C0B1

wet ice

human ... FTO(79068)

~58 kDa observed.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1