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Anti-GFP Antibody, clone 3F8.2
产品别名
Anti-GFP Antibody, clone 3F8.2
Green Fluorescent Protein
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light. |
| Specificity【特异性】 | Reacts with most common vertebrate proteins containing the GFP tag. The antibody reacts with the 28 kDa GFP protein. |
| Immunogen【免疫原】 | Full length GFP protein. Epitope: Full Length |
| Application【应用】 | Detect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB. Research Category Secondary & Control Antibodies Western Blot Analysis: 0.125 μg/mL - 1 μg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392). Research Sub Category Epitope Tags |
| Quality【质量】 | Routinely evaluated by Western Blot on GFP recombinant protein. |
| Physical form【外形】 | Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide. Format: Purified Protein G Purified |
| Analysis Note【分析说明】 | Control Western Blot: GFP recombinant protein |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 3F8.2, monoclonal |
| species reactivity | vertebrates |
| technique(s) | western blot: suitable |
| isotype【同位素/亚型】 | IgG1κ |
| UniProt accession no.【UniProt登记号】 | P42212 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | 28 kDa |
| Storage and Stability【储存及稳定性】 | Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
