Anti-Integrin α6 Antibody, clone MA6

32160702

NA.41

Reacts with the laminin receptor alpha-6beta-1 and alpha-6beta-4 complexes.

Immunoprecipitation, flow cytometry, immunohistochemistry in frozen tissue sections. Product does not detect alpha 6 in Western blot since the epitope most likely involves both alpha 6 and beta 1 integrin subunit. Product does not block alpha 6 integrin mediated cell adhesion to laminin. Activation of integrin signaling properties by MA6 has not been characterized. Immunoprecipitation of VLA-6 from lysate of 10E6 cell equivalent requires 2 μg of MA6. Recommended concentration for flow cytometry - 10 μg/mL, staining of frozen tissue sections - 20 μg/mL.

Optimal working dilutions must be determined by end user.

Cell lines tested by using MA6 to express (6 integrins include: mouse bone marrow-derived mast cells, melanoma cell line B16F1, squamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T lymphocytes, thymocytes and various T cell lines.
This Anti-Integrin α6 Antibody, clone MA6 is validated for use in FC, IP, IH for the detection of Integrin α6.

Liquid in 0.02M Phosphate buffer, pH 7.6, with 0.25M NaCl and 0.1% sodium azide.
Format: Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

rat

purified immunoglobulin

primary antibodies

MA6, monoclonal

mouse

Chemicon^®

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

IgG2κ

not suitable for Western blot

NM_000210.2
NM_001079818.1

P23229

wet ice

human ... ITGA6(3655)

Store at +2-8°C for up to 12 months.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2