Anti-Keratin K3/K76 抗体，克隆 AE5

32160702

NA.41

The antibody reacts with the 64 kDa polypeptide (cytokeratin 3; Keratin K3) of corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such this represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.

Rabbit corneal epithelial keratin

Anti-Keratin K3/K76 Antibody, clone AE5 is an antibody against Keratin K3/K76 for use in WB, IH, IH(P).
Immunohistochemistry on frozen sections: 1:50.

Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004).

Western blotting: 1:500.

Optimal working dilutions must be determined by the end user.

The monoclonal was purified by ion exchange chromatography is presented in phosphate buffered saline (pH 7.2) containing 0.09% sodium azide.
Ion-exchange chromatography
Format: Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified antibody

primary antibodies

AE5, monoclonal

human, rabbit, bovine, mouse

Chemicon^®

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

IgG1

NM_057088.2

P12035
Q01546

wet ice

human ... KRT3(3850)

64 kDa

For use within 1 month of purchase store at 2°-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2