Anti-Kindlin-1 Antibody, clone 4A5.14

32160702

NA.41

The Kindlin family is a group of focal adhesion proteins that have recently become a point of interest because of their ability to bind and activate integrins. Abnormalities in this ability have linked Kindlin to several diseases, both inherited and acquired, such as Kindler syndrome, cancer, and leukocyte adhesion deficiency. Specifically associated with Kindler syndrome, is the isoform Kindlin-1 which plays a key role in adhering the actin cytoskeleton to the extracellular matrix of basal keratinocytes. Kindlin-1 may also be involved in tumor growth by modulating TGF-beta 1 signaling (Shi, 2007). It is most widely expressed in the brain, kidney, colon, placenta, skeletal muscle, adrenal gland, and prostate.

The antibody recognizes Kindlin-1.

GST-tagged recombinant protein corresponding to human Kindlin-1.
Epitope: Unknown

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Papachristou, D.J., et al. (2007). Histopathology. 51(4):499–508.)

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH. (Papachristou, D.J., et al. (2007). Histopathology. 51(4):499–508.)
Research Category
Cell Structure
Anti-Kindlin-1 Antibody, clone 4A5.14 is an antibody against Kindlin-1 for use in WB, IC, IH.
Research Sub Category
Cytoskeleton

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Kindlin-1 in 10 µg of HeLa cell lysate.

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
HeLa cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

4A5.14, monoclonal

human

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

IgG2aκ

NP_060141

Q9BQL6

wet ice

human ... FERMT1(55612)

~ 70 kDa

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1