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Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11)
产品别名
Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11)
Anti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Ant-Murine Double Minute Chromosome-2
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 4B2C1.11, monoclonal |
| form【形式】 | liquid |
| does not contain【不包含】 | preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
| isotype【同位素/亚型】 | IgG1 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
| General description【一般描述】 | This Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MDM2 (Ab-5). Purified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein. Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 and MCF-7 cells and breast carcinoma tissue. |
| Immunogen【免疫原】 | recombinant, human MDM2 Human |
| Application【应用】 |  Immunoblotting (2 g/ml, chemiluminescence) Immunofluorescence (2.5 g/ml) Immunoprecipitation (1 g/reaction, see application references) Paraffin Sections (2.5 g/ml, heat pre-treatment required) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | In 50 mM sodium phosphate buffer, 50% glycerol, pH 7.5. |
| Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). |
| Analysis Note【分析说明】 | Positive Control A549 or MCF7 cells or breast carcinoma tissue |
| Other Notes【其他说明】 | Antibody should be titrated for optimal results in individual systems. Marchetti, A., et al. 1995. J. Pathol.175, 31. Barak, Y., et al. 1993. EMBO. J.12, 461. Ladanyi, M., et al. 1993. Cancer Res.53, 16. Leach, F.S., et al. 1993. Cancer Res.53, 2231. Oliner, J.D., et al. 1993. Nature362, 857. Momand, J., et al. 1992. Cell69, 1237. Oliner, J.D., et al. 1992. Nature358, 80. Fakharzadeh, S.S., et al. 1991. EMBO J.10, 1565. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
