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Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 488 Conjugate
| 产品编号： | 4110116 |
| 规格： | clone IF2, from mouse, ALEXA FLUOR® 488 |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 488 Conjugate
E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | E3 ubiquitin-protein ligase Mdm2 (UniProt Q00987; also known as Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2) is encoded by the MDM2 gene (Gene ID 4193) in human. MDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes p53 transcriptional activity by binding to and blocking p53 N-terminal trans-activation domain. MDM2 can also monoubiquitinate p53, thereby promoting p53 cytoplasmic localization to prevent p53 nuclear funtion. In addition, MDM2, when in complex with p300, also mediates p53 polyubiquitination, resulting in p53 proteosomal degradation. MDM2 overexpression is shown to inhibit DNA double-strand break repair mediated through its interaction with Nbs1 in a p53-independent manner. |
| Specificity【特异性】 | Clone IF2 recognizes the ~85/90 kDa (apparent MW) MDM2, as well as isoforms of ~57/60 kDa and ~74/76 kDa by immunoblotting using the unconjugated antibody (Cat. Nos. MABE340 & OP46). |
| Immunogen【免疫原】 | Epitope: Within amino acids 26-169. Recombinant human MDM2. |
| Application【应用】 | Research Sub Category Apoptosis - Additional This Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of MDM2. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MDM2 in A431 cells. The unconjugated antibody (Cat. Nos. MABE340 & OP46) is shown to be suitable also for immunohistochemistry and Western blotting applications. Research Category Epigenetics & Nuclear Function |
| Quality【质量】 | Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MDM2 in HeLa cells. |
| Physical form【外形】 | Protein G purified. Purified mouse monoclonal IgG2b antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide. |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
| Legal Information【法律信息】 | ALEXA FLUOR is a registered trademark of Life Technologies |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | ALEXA FLUOR® 488 |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | IF2, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable |
| isotype【同位素/亚型】 | IgG2b |
| NCBI accession no.【NCBI登记号】 | NP_002383 |
| UniProt accession no.【UniProt登记号】 | Q00987 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~85 kDa observed. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
