Anti-Mi-2β Antibody, clone 16G4

32160702

NA.41

CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.

Recombinant protein corresponding to Human Mi-2β.

Research Category
Epigenetics & Nuclear Function
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was used for ChIP. See Zhang et al. (2012).

ChIP-seq: representative lot of this antibody was used for ChIP-seq. See Zhang et al. (2012).
Research Sub Category
Chromatin Biology
This Anti-Mi-2β Antibody, clone 16G4 is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Mi-2β.

Evaluated by Western Blotting in a Jurkat cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β in a Jurkat cell lysate.

Purified mouse monoclonal IgG2aκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

16G4, monoclonal

human

ChIP: suitable (ChIP-seq)
western blot: suitable

IgG2aκ

NP_001264

Q14839

wet ice

human ... CHD4(1108)

~220 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2