Anti-monomethyl Histone H3 (Lys4) Antibody, clone RM140

32160702

NA.41

Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.

This antibody reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2) or trimethylated Lysine 4 (K4me3), or other methylations in Histone H3.

A linear peptide corresponding to human Histone H3 monomethylated at Lys4.

Multiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.
This Anti-monomethyl Histone H3 (Lys4) Antibody, clone RM140 is validated for use in Western Blotting, Multiplexing for the detection of monomethyl Histone H3.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins

Evaluated by Western Blotting in Recombinant HeLa protein and HeLa acid extract.

Western Blotting Analysis (WB): 0.5 μg/mL of this antibody detected monomethyl Histone H3 (Lys4) in HeLa acid extract.

Format: Purified
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Protein A purified

Concentration: Please refer to lot specific datasheet.

rabbit

100

primary antibodies

RM140, monoclonal

human

multiplexing: suitable
western blot: suitable

IgG

P84243

wet ice

human ... H3F3B(3021)

~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10-13 - German Storage Class 10 to 13