Anti-MRP1 Antibody, clone QCRL-1

32160702

NA.41

Reacts with a defined linear epitope (aa 918-924) of MRP1, a 190 kDa transmembrane

phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines

and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,

MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.

Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1 (Cole et al., 1992; Hipfner et al., 1994, 1999).

Anti-MRP1 Antibody, clone QCRL-1 detects level of MRP1 & has been published & validated for use in FC, WB, IC, IH, IH(P).
Western Blot: 0.2 μg/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996).

Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).

Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).

Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).

Immunoprecipitation (Hipfner, et al., 1994; 1996).

Optimal working dilutions must be determined by the end user.

Purified immunoglobulin. Liquid in PBS with 0.1% sodium azide.
Format: Purified

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

300

mouse

purified immunoglobulin

primary antibodies

QCRL-1, monoclonal

human

Chemicon^®

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

IgG1

NM_004996.3
NM_019862.2
NM_019898.2
NM_019899.2
NM_019900.2
NM_019901.1
NM_019902.1

P33527

wet ice

Store at 2-8ºC for up to 3 months. For longer term storage, aliquot and freeze at -20ºC.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2