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Anti-Na+/H+ Exchanger-1 Antibody, CT, clone 4E9
产品别名
Anti-Na+/H+ Exchanger-1 Antibody, CT, clone 4E9
NHE1
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Mammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue-specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated. |
Specificity【特异性】 | Na+/H+ exchanger, isoform NHE1 |
Immunogen【免疫原】 | MBP fusion protein containing the entire C-terminal, hydrophylic domain of porcine NHE1. Epitope: C-terminus |
Application【应用】 | Anti-Na+/H+ Exchanger-1 Antibody, C-terminus, clone 4E9 detects level of Na+/H+ Exchanger-1 & has been published & validated for use in WB. Research Category Neuroscience Research Sub Category Ion Channels & Transporters Works poorly for immunohistochemistry and immunocytochemistry. Not recommended for immunoprecipitation. Immunoblotting: 1:1,000 dilution used on a previous lot. Optimal working dilutions must be determined by the user. |
Quality【质量】 | Routinely evaluated by Western Blot on Human Kidney lysates. Western Blot: 1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates. |
Physical form【外形】 | Purified mouse monoclonal IgG1 liquid in buffer containing 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide Format: Purified Protein A purified |
Analysis Note【分析说明】 | Control Kidney tissue (basolateral membrane) |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 4E9, monoclonal |
species reactivity | amphibian, vertebrates (including including fish, amphibians, birds and mammals), avian, fish |
species reactivity (predicted by homology) | mammals, mouse, rat |
manufacturer/tradename | Chemicon® |
technique(s) | western blot: suitable |
isotype【同位素/亚型】 | IgG1 |
suitability【适用性】 | not suitable for immunoprecipitation |
NCBI accession no.【NCBI登记号】 | NM_003047.2 |
UniProt accession no.【UniProt登记号】 | P19634 |
shipped in【运输】 | wet ice |
Gene Information | human ... SLC9A1(6548) |
产品说明
Target description【目标描述】 | ~100-110 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8ºC from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |