Anti-NCAM1 Antibody, clone 11G7.1

32160702

NA.41

The Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulin proteins by structure. The family contains over a 100 members, which include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural evelopment. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.

This antibody recognizes the cytoplasmic domain of NCAM1.

Epitope: cytoplasmic domain
GST-tagged recombinant protein corresponding to the cytoplasmic domain of mouse NCAM1.

This NCAM1 antibody is validated for use in WB & IHC for the detection of the NCAM1 protein.
Research Category
Neuroscience
Western Blot Analysis: 0.5 µg/mL from a representative lot detected NCAM1 in 10 µg of mouse brain tissue lysate.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected NCAM1 in rat hippocampus and rat cerebral cortex tissues.
Research Sub Category
Developmental Signaling

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NCAM1 in 10 µg of rat brain tissue lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
Rat brain tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

11G7.1, monoclonal

mouse, rat

immunohistochemistry: suitable
western blot: suitable

IgG1κ

NP_001074914

P13595

wet ice

~180 kDa observed. Uniprot describes 3 isoforms produced by alternative splicing at ~94 kDa, ~80 kDa, and ~67 kDa The calculated molecular weight of this protein is 120 kDa, but can be observed between ~105-200 kDa due to high glycosylation (Patel, K., et al. (1991). Br J Cancer Suppl. 14:20-13).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1