Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324

32160702

NA.41

Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules: N-CAM, myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts

Monoclonal antibody against neural cell adhesion molecule L1 from rat-rat hybrid cells.

Rats were immunized with a glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.

This Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1.
Western blotting:
20 μg/mL was used on a previous lot.

Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.

Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.

Optimal working dilutions must be determined by end user.

Protein A purified
Rat monoclonal in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Format: Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

rat

purified antibody

primary antibodies

324, monoclonal

mouse, rat

Chemicon^®

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

IgG

NP_032504.3

P11627

wet ice

human ... L1CAM(3897), VCAM1(7412)

120-140 kDa

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2