Anti-Neurexin-1-alpha Antibody

32160702

NA.41

Neurexins are heavily glycosylated and processed neuronal transmembrane cell surface proteins likely involved in cell recognition and adhesion (see review by Missler & Sudhof, 1998). Antibody Anti-Neurexin-1-alpha (ABN35) was made against a peptide immunogen corresponding to a stretch of amino acids in the extracellular domain of human Neurexin-1-alpha (Uniprot Q9ULB1). Although the theoretical full length of Neurexin-1-alpha is 161 kDa, the protein detected in human brain lysates may show a band around 160 kDa and additional bands around 45 kDa as a possible result of the posttranslational processing previously reported.

This antibody recognizes Neurexin-1-alpha at the extracellular laminin G-like 3 domain.

Epitope: Extracellular laminin G-like 3 domain
KLH-conjugated Linear peptide corresponding to human Neurexin-1-alpha at the extracellular laminin G-like 3 domain.

Detect Neurexin-1-alpha using this Anti-Neurexin-1-alpha Antibody validated for use in WB, IH(P).
Research Category
Neuroscience
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Neurexin-1-alpha in human brain tissue.
Research Sub Category
Adhesion (CAMs)

Evaluated by Western Blot in human cerebellum tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Neurexin-1-alpha on 10 µg of human cerebellum tissue lysate.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
Human cerebellum tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

mouse, human, rat

rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NP_004792

Q9ULB1

wet ice

human ... NRXN1(9378)

~ 45 kDa Observed At higher concentrations or longer exposures, in certain lysates, a band at ~150kD can be seen that corresponds to the expected full length Mw.
UniProtKB/Swiss-Prot Q9ULB1 (NRX1A_HUMAN) entry describes 3 isoforms produced by alternative promoter usage and alternative splicing. Reference (Accolade/Related Documents) TIG JANUARY 1998 VOL. 14 No. 1 indicates that multiple neurexin isoforms are generated from three genes by alternative promoters and alternative splicing.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1