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Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2
产品别名
Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2
Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known. |
| Specificity【特异性】 | Reactivity to other species has not been determined. This antibody reacts with the intracellular protein, Oct-4 and detects the Oct-4A isoform. |
| Immunogen【免疫原】 | Recombinant human Oct-4 protein, a.a. 1-145. |
| Application【应用】 | This Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2 is validated for use in IC, FC, WB for the detection of OCT-4 [POU5F1]. Western Blotting: Recommended working dilution is 1 - 2 µg/mL. Flow Cytometry: Recommended working dilution is 2 µg/mL. Immunocytochemistry: Recommended working dilution is 1:100 – 1: 200. Research Sub Category Pluripotent & Early Differentiation Research Category Stem Cell Research |
| Linkage【联系】 | Replaces: MAB4305 |
| Physical form【外形】 | Protein G sepharose column Format: Purified Purified mouse monoclonal IgG1κ in a buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% NaN3. |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 7F9.2, monoclonal |
| species reactivity | mouse, human |
| technique(s) | flow cytometry: suitable immunocytochemistry: suitable western blot: suitable |
| input | sample type: mouse embryonic stem cell(s) sample type induced pluripotent stem cell(s) sample type: human embryonic stem cell(s) |
| isotype【同位素/亚型】 | IgG1κ |
| NCBI accession no.【NCBI登记号】 | NP_976034 |
| UniProt accession no.【UniProt登记号】 | P20263 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~39 kDa |
| Storage and Stability【储存及稳定性】 | Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
