您的位置：首页 > 产品中心 > Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7)
Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7)
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | DO-7, monoclonal |
| form【形式】 | liquid |
| does not contain【不包含】 | preservative |
| species reactivity | bovine, monkey, human |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
| isotype【同位素/亚型】 | IgG2b |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
| General description【一般描述】 | Recognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue. Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53. This Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-12) (Pantropic). |
| Immunogen【免疫原】 | recombinant, human p53 expressed in E. coli Epitope: within amino acids 37-45 of p53 |
| Application【应用】 |   Frozen Sections (1-2 g/ml) Immunoblotting (1-2 g/ml) Immunofluorescence (0.5 g/ml) Immunoprecipitation (2 g/mg lysate) Paraffin Sections (1-2 g/ml) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Analysis Note【分析说明】 | Positive Control SKBR-3 or MDA-231 cells or colon carcinoma tissue |
| Other Notes【其他说明】 | Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems. Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855. Lane, D.P. 1992. Nature358, 15. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
