Anti-Pan DIDO Antibody

32160702

Death-inducer obliterator 1 (UniProt Q9BTC0; also known as DATF-1, Death-associated transcription factor 1, DIO-1, hDido1) is encoded by the DIDO1 (also known as C20orf158, DATF1, KIAA0333) gene (Gene ID 11083) in human. The DIDO1 gene encodes four alternatively spliced variants (DIDO1/isoform 2, DIDO2/isoform 1, DIDO3/isoform 4, isoform a/isoform 3) implicated to be involved in apoptosis and development. The longest and most widely expressed DIDO3 is assigned as the canonical isoform. DIDO3 is a nuclear protein that forms part of the spindle assembly checkpoint (SAC) and is necessary for correct chromosome segregation in somatic and germ cells. Dido3-knockout compromises differentiation of murine embryonic stem cells (mESCs) in vitro and of epiblast cells in vivo, resulting in early embryonic death at around day 8.5 of gestation. On the other hand, exogenous expression of Dido1 isoform in mESCs is reported to inhibit LIF withdrawal-induced differentiation, while Dido1 knockdown facilitate mESCs differentiation.

This polyclonal antibody detects an N-terminal epitope present in all four spliced isoforms of human DIDO1 reported by UniProt (Q9BTC0).

KLH-conjugated linear peptide corresponding an N-terminal sequence of human DIDO1.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected DIDO immunoreactivity in human kindey, human prostate cancer and human cerebellum tissue.
This Anti-Pan DIDO Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of DIDO.

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected DIDO isoforms in 10 µg of HepG2 cell lysate.

Affinity purified.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

nonhuman primates (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology)

immunohistochemistry: suitable
western blot: suitable

NP_071388

Q9BTC0

human ... DIDO1(11083)

~260/130/79/70/66 observed. 59.46 kDa (isoform 2; DIDO1), 129.2 kDa (isoform 1; DIDO2), 243.9 kDa (isoform 4; DIDO3), 61.35 kDa (isoform 3; isoform a) calculated. Uncharacterized band(s) may appear in some lysates.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1