Anti-phospho Histone H3 (Thr11) Antibody, clone N123/48

32160702

NA.41

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation.

Linear peptide corresponding to rat Histone H3 phosphorylated at Thr11.

Immmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected phospho Histone H3 (Thr11) in HeLa cells.

Dot Blot Analysis: A 1:5,000 dilution from a representative lot detected phospho Histone H3 (Thr11) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

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This Anti-phospho Histone H3 (Thr11) Antibody, clone N123/48 is validated for use in western blotting, ICC & dot blot for the detection of phospho Histone H3 (Thr11).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blotting in Colcemid treated and untreated HeLa acid extract.

Western Blotting Anlaysis: 1:5,000 dilution of this antibody detected phospho Histone H3 (Thr11) in Colcemid treated HeLa acid extract and not in untreated HeLa acid extract.

Replaces: MABE468

Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

100

mouse

purified immunoglobulin

primary antibodies

N123/48, monoclonal

human

mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

0.5 mg/mL

dot blot: suitable
immunocytochemistry: suitable
western blot: suitable

IgG1

NP_001013074

Q6LED0

wet ice

~17 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1