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Anti-phospho-NKCC1 Antibody (Thr212/Thr217)
产品别名
Anti-phospho-NKCC1 Antibody (Thr212/Thr217)
phospho-NKCC1 Thr212/Thr217, Solute carrier family 12 member 2, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | The Na-K-Cl cotransporter 1 (NKCC1) is a membrane protein coded by the SLC12A2 gene and functions in cellular sodium/potassium/chloride transport and homeostasis. It is widely distributed throughout the body and serves important functions, especially in liquid-secreting glands. Phosphorylation of Thr212/Thr217 in the intracellular N-terminal domain of NKCC1 is correlated with NKCC1 activation. |
| Specificity【特异性】 | Specific for mature form of phosphorylated NKCC1. |
| Immunogen【免疫原】 | Linear peptide corresponding to human phospho-NKCC1 Thr212/Thr217. |
| Application【应用】 | Western Blotting Analysis: A representative lot detected phospho-NKCC1 Thr212/Thr217 in GMB43 and GBM5 cell lysate (Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012). JBC. 1-31). Immunofluorescence Analysis: A representative lot detected phospho-NKCC1 Thr212/Thr217 in GBM43 human tissiue (Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012). JBC. 1-31). Research Category Signaling Research Sub Category Signaling Neuroscience Anti-phospho-NKCC1 Thr212/Thr217 is an antibody against phospho-NKCC1 for use in Western Blotting, Immunofluorescence. |
| Quality【质量】 | Evaluated by Western Blotting in GMB43 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-NKCC1 Thr212/Thr217 in 10 µg of GMB43 cell lysate. |
| Physical form【外形】 | Rabbit polyclonal serum with 0.05% sodium azide. Unpurified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | serum |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| species reactivity | human |
| technique(s) | immunofluorescence: suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_001037 |
| UniProt accession no.【UniProt登记号】 | P55011 |
| shipped in【运输】 | dry ice |
| Gene Information | human ... SLC12A2(6558) |
产品说明
| Target description【目标描述】 | ~250 kDa observed. Likely a dimer of mature form of NKCC1, NKCC1 is glycosylated differently in different cells, leading to uncharacterized band(s) in some lysates. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
