Anti-Protein-arginine deiminase 4 Antibody, clone 10F1

32160702

NA.41

Protein-arginine deiminase type-4 (PAD4; formerly PAD5) is one of five PAD (1-4, 6) homologues that belong to the superfamily of multidomain amidinotransferases. The PAD enzymes catalyze deiminating reactions in which guanidinium is hydrolyzed to peptidylcitrulline. PAD4 is a calcium-regulated nuclear-localized enzyme. It modifies arginine residues on histones H2A, H3, and H4 (H2 Arg3; H3 Arg2, H3 Arg8, H3 Arg17, H3 Arg26; and H4 Arg3). Several studies have suggested that PAD4-mediated citrullination has important implications for gene regulation. PAD4 may perform deimination at sites overlapping with arginine methylation, and antagonize the transcription-promoting effects of methylating enzymes such as CARM1 and PRMT1. PAD4 may therefore inhibit gene expression, most notably expression of cell-cycle regulating p53 genes such as p21. Previous studies have suggested that PAD4 may play central role in the development of cancer.

GST-tagged recombinant protein corresponding to human Protein-arginine deminiase 4.

Use Anti-Protein-arginine deiminase 4 Antibody, clone 10F1 (mouse monoclonal antibody) validated in WB, IP to detect PAD-4 also known as HL-60 PAD, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV.
Research Category
Epigenetics & Nuclear Function
Immunoprecipitation Analysis: A representative lot immunoprecipitated Protein-arginine deminiase 4 in IP (Prof. K. Mowen, Department of Chemical Physiology, The Scripps Research Institute-California Campus.).

Chromatin Immunoprecipitation Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated Protein-arginine deminiase 4 in ChIP-Seq (Price, D. H., et al. (2011). Mol Cell. 45(1):38-50.).
Research Sub Category
Chromatin Biology

Evaluated by Western Blot in DMSO treated and untreated HL-60 cell lysates.

Western Blot Analysis: 0.25 µg/mL of this antibody detected Protein-arginine deminiase 4 in 10 µg DMSO treated and untreated HL-60 cell lysates.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
DMSO treated and untreated HL-60 cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified antibody

primary antibodies

10F1, monoclonal

human

ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

NP_036519

Q9UM07

wet ice

~74 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK 1