Anti-PTP1B Antibody, clone FG6-1G

32160702

NA.41

PTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and may be a potential therapeutic target for diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.

This antibody recognizes the catalytic domain of PTP1B.

Recombinant protein corresponding to the catalytic domain of human PTP1B.
Epitope: Catalytic domain

Research Sub Category
Cytoskeletal Signaling
Research Category
Signaling
Western Blot Analysis: A representative lot was used to detect PTP1B in Jurkat cell lysate.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected PTP1B in A431 cell lysate (Gulati, P. et al. (2004). EMBO Rep. 5(8):812-817.).
Anti-PTP1B Antibody, clone FG6-1G is an antibody against PTP1B for use in Western Blotting, Immunoprecipitation.

Evaluated by Western Blot in TF-1 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PTP1B in 10 µg of TF-1 cell lysate.

Replaces: 04-1140

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

Control
TF-1 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

FG6-1G, monoclonal

human

immunoprecipitation (IP): suitable
western blot: suitable

IgG2aκ

NP_002818

P18031

wet ice

human ... PTPN1(5770)

~50 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1