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Anti-Pyrin
| 产品编号： | 4113360 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 25 μL，100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Pyrin
Marenostrin
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Pyrin (UniProt: O15553; also known as Marenostrin) is encoded by the MEFV (also known as MEF, TRIM20) gene (Gene ID: 4210) in human. Pyrin is a homotrimeric protein that is involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. It organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Pyrin acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence it may prevent excessive IL-1B- and IL-18-mediated inflammation. Pyrin is expressed in peripheral blood leukocytes, particularly in mature granulocytes and to a lesser extent in monocytes, but not in lymphocytes. It is also detected in spleen, lung and muscle, probably as a result of leukocyte infiltration in these tissues. Its expression is also detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines. Three isoforms of Pyrin have been described that are produced by alternative splicing. Isoform 1 associates with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles. In pre-apoptotic cells, it co-localizes with PYCARD/ASC in large specks (inflammasome). Isoform 2 is shown to be localized to the nucleus. |
| Specificity【特异性】 | This rabbit polyclonal antibody detects Pyrin in human cells. It targets an epitope within 15 amino acids from the N-terminal region. |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Pyrin, isoform 1. |
| Application【应用】 | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Pyrin in human tonsil, human cerebral cortex, human skeletal muscle, and human pancreatic cancer tissues. Anti-Pyrin, Cat. No. ABF1169, is a rabbit polyclonal antibody that detects human Pyrin and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Quality【质量】 | Evaluated by Western Blotting in THP-1 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Pyrin in 10 µg of THP-1 cell lysate. |
| Other Notes【其他说明】 | Affinity Purified |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| packaging【包装】 | antibody small pack of 25 μL |
| technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_000234 |
| UniProt accession no.【UniProt登记号】 | O15553 |
| shipped in【运输】 | ambient |
产品说明
| Target description【目标描述】 | ~82 kDa observed; 86.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
