Anti-Rab21 Antibody

32160702

NA.41

RAB21 is a member of the small GTPase superfamily that is critically important in the recycling of integrin protein receptor complexes and their internalization. Thus, RAB21 is important in cell adhesion and migration of cells, as well as the proper completing of cytokinesis which requires particular care with adherent cells to coordinate the release and recycling of integrin and their release from the ECM matrix during cytokinesis. RAB21 is primarily a cytoplasmic or cytoplasmic organelle localized protein and its precise location will vary with cell cycle and cell division phases. RAB21 is widely expressed particularly in cells in the gut and capillaries where cell division and cell morphological changes are common.

GST-tagged recombinant protein corresponding to Mouse Rab21.

Research Sub Category
Apoptosis - Additional
Western Blot Analysis: A 1:1000 dilution of a representative lot of this antibody detected Rab21 in A431, C2C12, HEK293, HeLa, HepG2, Jurkat, L6, Mouse brain, NIH/3T3, PC12, PC3, and RAW264.7 cell lysates. This antibody did not detect Rab21 in Human placenta or Huvec cell lysates.

Western Blot Analysis: A representative lot of this antibody detected Rab21 in melanocyte membrane compartment lysate (Ohbayashi et al., (2012) Mol Biol Cell. 23(4):669-678).
This Anti-Rab21 Antibody is validated for use in Western Blotting for the detection of Rab21.
Research Category
Apoptosis & Cancer

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected Rab21 in 10 µg of PC12 cell lysate.

Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Antigen Affinity Purified

Concentration: Please refer to lot specific datasheet.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, rat, mouse

western blot: suitable

NP_055814

Q9UL25

wet ice

human ... RAB21(23011)

~24 kDa observed.
Uncharacterized bands may be obeserved in some lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1