Anti-Rab27B Antibody

32160702

NA.41

RAB27B, also known as C25KG, is a member of the Rab GTPase superfamily. RAB27B acts as a lipid anchoring protein that is prenylated and works in vesicular fusion and protein trafficking within cells. The protein is most highly expressed in testis but can be expressed in other cell types as well. Because Rab27B is widely expressed in various tissues together with Rab27A and RAB27B has been found to have the ability to bind all of the Rab27A effectors that have been tested, Rab27A and Rab27B were initially thought to function redundantly by sharing common Rab27 effectors. However, recent evidence has indicated that by interacting with different Rab27 effectors Rab27A and Rab27B play different roles in special types of secretion (e.g. exosome secretion and mast cell secretion) even within the same cell type. Rab27B is particularly important for regulating protein secretion by pancreatic acinar cells, for instance.

GST-tagged recombinant protein corresponding to human Rab27B.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Rab27B in 10 µg of C2C12, mouse brain, and PC3 lysate.
Western Blotting Analysis: A representative lot detected Rab27B in rat parotid gland lysate (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Rab27B in flg-Rab27B (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Immunohistochemistry Analysis: A representative lot detected Rab27B in rat parotid acinar cells (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
This Anti-Rab27B Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunohistochemistry for the detection of Rab27B.

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rab27B in 10 µg of mouse brain tissue lysate.

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

Concentration: Please refer to lot specific datasheet.

rabbit

100

serum

primary antibodies

polyclonal

rat, mouse, human

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NP_004154.2

O00194

wet ice

human ... RAB27B(5874)

~27 kDa observed. Uncharacterized band(s) may appear in some lysates.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1