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Anti-RBP-Jk, clone 1F1 Antibody
产品别名
Anti-RBP-Jk, clone 1F1 Antibody
Recombining binding protein suppressor of hairless, CBF-1, J kappa-recombination signal-binding protein, RBP-J kappa, RBP-J, RBP-JK, Renal carcinoma antigen NY-REN-30
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | RBP-jk, Recombinant signal binding protein for immunoglobin kappa J region, is a human homolog for the Drosophila gene, suppressor of hairless. RBP-jk is located in the nucleus and is a 60 kDa monomeric protein that is used as a promoter region in notch signaling. Notch signaling is important in cell to cell communication which allows "a differentiating cell to suppress the same differentiation fate in a neighboring cell (the receiving cell). This ensures that the correct number of cells are distributed to distinct cell fates." When RBP-jk is not associated with Notch proteins, it acts as a transcriptional repressor. A disease associated with RBP-jk is the Epstein Barr virus. |
| Immunogen【免疫原】 | Recombinant protein corresponding to human RBP-Jk. |
| Application【应用】 | Research Sub Category Nuclear Receptors This Anti-RBP-Jk, clone 1F1 Antibody is validated for use in Immunoprecipitation and Chromatin Immunoprecipitation (ChIP) and Electrophoretic Mobility Shift Assay for the detection of RBP-Jk. Research Category Epigenetics & Nuclear Function Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using Lymphoblastoid cell line. Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using DG75 cells. Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using Hippocampal NSC′s (Ehm, O., et al. (2010). Journal of Neuroscience. 30(41):13794-18707). Electrophoretic Mobility Shift Assay: A representative lot detected RBP-Jk using Hippocampal lysate (Ehm, O., et al. (2010). Journal of Neuroscience. 30(41):13794-18707). |
| Quality【质量】 | Evaluated by Immunoprecipitation in HeLa cell lysate. Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated RBP-Jk in 0.5 mg of HeLa cell lysate. |
| Physical form【外形】 | Purified rat IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | rat |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 1F1, monoclonal |
| species reactivity | Drosophila, human |
| technique(s) | ChIP: suitable electrophoretic mobility shift assay: suitable immunoprecipitation (IP): suitable |
| isotype【同位素/亚型】 | IgG2aκ |
| NCBI accession no.【NCBI登记号】 | NP_005340 |
| UniProt accession no.【UniProt登记号】 | Q06330 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~55 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
