Anti-RGS4 Antibody

32160702

NA.41

Regulator of G-protein signaling 4 (RGS4) is a GTPase activating protein belonging to the RGS family. This is a group of proteins known to act as GTPase activating proteins (GAPs), in order to regulate G protein signaling. RGS4 is highly expressed throughout the brain with highest expression observed in the prefrontal cortex, and some expression found in peripheral tissues such as the heart. RGS4 acts as a signal transduction inhibitor by inactivating G protein alpha subunits through the increase of their GTPase activity. Also, RGS4 serves to enhance Gi/o and Gq/11 protein GTPase activity and to expedite the conclusion of GPCR signaling. Various studies indicate a potential role for RGS4 in the regulation of various neurotransmitter systems, including those with putative involvement in schizophrenia. Mutations in the RGS4 gene lead to a lower presence of RGS4, linked to both Schizophrenia and Alzheimer’s symptoms.

Recombinant protein corresponding to rat RGS4.

Western Blot Analysis: 1:1,000 dilution from a representative lot detected RGS4 on 10 µg of recombinant RGS4.
Research Category
Cell Structure

Signaling
Research Sub Category
Cytoskeletal Signaling

G-proteins
Detect RGS4 using this Anti-RGS4 Antibody validated for use in WB.

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 1:1000 dilution of the antibody detected RGS4 in 10 µg of PC12 cell lysate.

Unpurified
Rabbit polyclonal serum containing 0.05% sodium azide.

Control
PC12 cell lysate

rabbit

100

serum

primary antibodies

polyclonal

rat

western blot: suitable

IgG

NP_058910

P49799

wet ice

human ... RGS4(5999)

~ 23 kDa MW observed. A non-specific band appears at ~ 12 kDa

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1