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Anti-RhoGAP1 Antibody

产品编号:4113684
规格:from rabbit, purified by affinity chromatography
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4113684100 μG4590
产品别名

Anti-RhoGAP1 Antibody

Rho GTPase-activating protein 1, CDC42 GTPase-activating protein, GTPase-activating protein rhoOGAP, Rho-related small GTPase protein activator, Rho-type GTPase-activating protein 1, p50-RhoGAP, RhoGAP1

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
RhoGAP1, also known as Rho GTPase-activating protein 1, CDC42 GTPase-activating protein, Rho-related small GTPase protein activator, Rho-type GTPase-activating protein 1, or p50-RhoGAP, and encoded by the gene ARHGAP1/CDC42GAP/RHOGAP1, is an important GTPase activator for the Rho, Rac, and CDC42 GTP binding proteins, converting them to the putatively inactive GDP-bound state. CDC42 is the preferred substrate for RhoGAP1. Such GTPase-activating proteins or GAPs play an important role in signal transduction pathways regulated by GTP-binding proteins. In addition to acting as down-regulators of GTPases, there is growing evidence that they also act as effector molecules required for downstream signaling. RhoGAP1 is found in multiprotein complexes in the cytoplasm in most cells. Recent research has shown RhoGAP1 to be particularly important during epithelial to mesenchymal morphogenesis and for carcinoma metastasis. Rho-ROCK signaling is essential for cell apical detachment and the generation of motility. RhoGAP1 restricts Rho activation to apical areas and thus helps guide the detachment process and thus is a target of much developmental and cancer research.
Immunogen【免疫原】
GST-tagged recombinant protein corresponding to human RhoGAP1.
Application【应用】
Anti-RhoGAP1 Antibody is an antibody against RhoGAP1 for use in western blotting & ICC.
Research Sub Category
Cell Cycle, DNA Replication & Repair
Research Category
Signaling
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected RhoGAP1 in 10 µg of A431, NIH/3T3, and PC-12 cell lysates.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected RhoGAP1 in HeLa cells (Schiel, J. A., et al. (2012). Nat Cell Biol. 14(10):1068-1078.).
Quality【质量】
Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected RhoGAP1 in 10 µg of HeLa cell lysate.
Physical form【外形】
Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
affinity isolated antibody
antibody product type
primary antibodies
clone【克隆】
polyclonal
purified by【纯化方式】
affinity chromatography
species reactivity
human, mouse, rat
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.【NCBI登记号】
NP_004299
UniProt accession no.【UniProt登记号】
Q07960
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
~50 kDa observed
Storage and Stability【储存及稳定性】
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 2
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