Anti-RIG-I, clone 1C3 Antibody

32160702

NA.41

Retinoic acid-inducible gene 1 protein (RIG-1) is a cytoplasmic DExD/H box RNA helicase belonging to the helicase family and containing 2 CARD domains within the N-terminus, a repressor domain (RD), a helicase ATP-binding domain and a helicase C-terminal binding domain. RIG-1 is able to bind the 5’-triphosphate RNA of single or double-stranded viral RNA. After recognizing and binding viral RNA, RIG-1 protein initializes type I IFN production, and within fibroblasts and dendritic cells plays a central role in the regulation of type I IFN production in response to viral presence. There are four known isoforms resulting from alternative splice variants.

Recombinant protein corresponding to human RIG-I.

Immunoprecipitation Analysis: A representative lot immunoprecipitated RIG-1 in A-549 infected with SeV cell lysate (Baum, A., et al. (2011). PNAS. 108(7):16303-16308).
Immunoprecipitation Analysis: A representative lot immunoprecipitated RIG-1 in A-459 lung carcinoma cell lysate (Nistal-Villan, E., et al. (2010). J. Biol. Chem. 285:20252-20261).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected RIG-I in HeLa cells (Courtesy of Maite Sanchez).
Research Sub Category
Immunoglobulins & Immunology
This Anti-RIG-I, clone 1C3 Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of RIG-I.
Research Category
Inflammation & Immunology

Evaluated by Western Blotting in A-549 infected with SeV cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected RIG-1 in 10 µg of A-549 infected with SeV cell lysate.

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

1C3, monoclonal

human

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1λ

NP_055129

O95786

wet ice

~110 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1