广州伟伯科技有限公司
您的位置:首页 > 产品中心 > Anti-Ring Finger Protein 213 (RNF213)
产品搜索:

Anti-Ring Finger Protein 213 (RNF213)

产品编号:4113709
规格:from rabbit
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4113709100 μG4510
产品别名

Anti-Ring Finger Protein 213 (RNF213)

E3 ubiquitin-protein ligase RNF213, ALK lymphoma oligomerization partner on chromosome 17, Mysterin, RING finger protein 213, RING-type E3 ubiquitin transferase RNF213

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
E3 ubiquitin-protein ligase RNF213 (UniProt: Q63HN8; also known as ALK lymphoma oligomerization partner on chromosome 17, Mysterin, RING finger protein 213, RING-type E3 ubiquitin transferase RNF21) is encoded by the RNF213 (also known as ALO17, C17orf27, KIAA1554, KIAA1618, MYSTR) gene (Gene ID: 57674) in human. RNF213 is a widely expressed, homooligomeric, cytoplasmic protein that has E3 ubiquitin-protein ligase and AAA+ ATPase activity. It contains a zinc-finger domain (aa 3997-4036) that is required for its ubiquitin-protein ligase activity. RNF213 is shown to be involved in angiogenesis and in non-canonical Wnt signaling pathway in vascular development. Four isoforms of RNF213 have been described that are produced by alternative splicing. RNF213 ligase activity is negatively regulated by PTP1B in HER2+ breast cancer cells and RNF213 knockdown is shown to reverse the effects of PTP1B deficiency on alpha-keto-dependent dehydrogenases, non-mitochondrial oxygen consumption, and hypoxia-induced death of HER2+ BC cells. RNF213 activity is down-regulated by let-7c miRNA, which binds to the 3′-UTR transcript of RNF213. Microbial infection leading to induction of pro-inflammatory cytokines are shown to up-regulate its activity. Defects in RNF213 gene are known to cause Moyamoya disease 2, a progressive cerebral angiopathy characterized by bilateral intracranial carotid artery stenosis and telangiectatic vessels in the region of the basal ganglia, which can lead to transient ischemia and/or rupture of the collateral vessel. (Ref.: Banh, RS et al (2016). Nat. Cell Biol. 18(7); 803-813).
Specificity【特异性】
This rabbit polyclonal antibody detects human and mouse Ring Finger Protein 213.
Immunogen【免疫原】
11 GST-tagged recombinant fragments from human Ring Finger Protein 213.
Application【应用】
Anti-Ring Finger Protein 213 (RNF213), Cat. No. ABC1391, is a rabbit polyclonal antibody that detects E3 ubiquitin-protein ligase RNF213 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Immunoprecipitation Analysis: A representative lot detected Ring Finger Protein 213 (RNF213) in BT474, MDA-MB-361, and HCC1954 cells (Banh, R.S., et. al. (2016). Nat Cell Biol. 18(7):803-13).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Ring Finger Protein 213 (RNF213) in HUVEC transfected with siRNA213 (Courtesy of Dr Akio Koizumi at Kyoto University).

Immunocytochemistry Analysis: A representative lot detected Ring Finger Protein 213 (RNF213) in HeLa cells treated with RNF213 siRNA (Hitomi, T., et. al. (2013). Biochem Biophys Res Commun. 438(1):13-9).

Western Blotting Analysis: A representative lot detected Ring Finger Protein 213 (RNF213) in Western Blotting applications (Hitomi, T., et. al. (2013). Biochem Biophys Res Commun. 438(1):13-9; Banh, R.S., et. al. (2016). Nat Cell Biol. 18(7):803-13; Kobayashi, H., et. al. (2015). J Am Heart Assoc. 4(7)).
Quality【质量】
Evaluated by Western Blotting in HEK293T cells transfected with 3XFlag RNF213 wild-type.

Western Blotting Analysis: 1 µg/mL of this antibody detected Ring Finger Protein 213 (RNF213) in HEK293T cells transfected with 3XFlag RNF213 wild-type.
Physical form【外形】
Protein A purified
Format: Purified
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
human, mouse
packaging【包装】
antibody small pack of 25 μg
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype【同位素/亚型】
IgG
NCBI accession no.【NCBI登记号】
NP_001243000
UniProt accession no.【UniProt登记号】
Q63HN8
shipped in【运输】
ambient
Gene Information
human ... RNF213(57674)
产品说明
Target description【目标描述】
~596 kDa observed; 591.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
广州伟伯科技有限公司 版权所有 CopyRight ©2006-2024, All Rights Reserved
工信部备案号:粤ICP备08114744号   Page Run Time: 0.9076